Real-time viability assay based on 51Cr retention in adherent cells

Real-time viability assay based on 51Cr retention in adherent cells.

The chromium (51Cr) release assay has been widely used for viability measurements, even though it has major disadvantages such as high manual workload and poor time resolution. By the use of LigandTracer 51Cr release viability measurements on adherent cells can be significantly simplified and improved. LigandTracer enables a time-resolved detection of 5SCr in target cells, with the result that ...

Clonogenic assay: adherent cells.

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 1956. Apart from documenting the method, the initial landmark study generated the first radiation-dose response curve for X-ray irradiated mammalian (HeLa) cells in culture. Basically, the clonogenic assay enables an assessment of the differences in ...

Real-Time and Dynamic Monitoring of Cell Proliferation and Viability for Adherent Cells

Real-Time Control for Autonomous Racing Based on Viability Theory

In this paper we consider autonomous driving of miniature race cars. The viability kernel is used to efficiently generate finite look ahead trajectories that maximize progress while remaining recursively feasible with respect to static obstacles (e.g., stay inside the track). Together with a low level model predictive controller, this method makes real time autonomous racing possible. The viabi...

A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient

Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...